tapi 2  (MedChemExpress)

Journal: International Journal of Molecular Sciences

Article Title: Involvement of ADAM17-Klotho Crosstalk in High Glucose-Induced Alterations of Podocyte Function

doi: 10.3390/ijms26020731

Figure Lengend Snippet: Elevation of ADAM17 protein levels and activity in renal tissues and urine in healthy rats and rats with STZ-induced diabetes and effects of high glucose concentrations on ADAM17 activity in podocytes. ( a ) Increase in ADAM17 protein levels (brown staining) in renal tissues ( n = 11). Scale bar = 100 μm. ( b ) Increase in ADAM17 protein levels in diabetic glomeruli ( n = 13). ( c ) ADAM17 activity ( n = 6) was elevated in urine in diabetic rats. ( d ) ADAM17 protein expression in podocytes ( n = 6). ( e ) Decrease in ADAM17 activity in podocyte lysates ( n = 4). ( f ) Increase in extracellular ADAM17 activity in podocytes ( n = 4). The results are expressed as the mean ± SD. ** p < 0.01, * p < 0.05. Ctrl, control; ns, non-statistically significant; STZ, streptozotocin; SG, standard glucose; HG, high glucose.

Article Snippet: The ADAM17 inhibitor N -( R )-(2-[hydroxyaminocarbonyl]methyl)-4-methylpentanoyl-L-naphthylalanyl-L-alanine amide (TAPI-0) was purchased from Merck (catalog no. SML1292).

Techniques: Activity Assay, Staining, Expressing, Control

Journal: International Journal of Molecular Sciences

Article Title: Involvement of ADAM17-Klotho Crosstalk in High Glucose-Induced Alterations of Podocyte Function

doi: 10.3390/ijms26020731

Figure Lengend Snippet: Inhibition of ADAM17 improved albumin permeability in the glomeruli of diabetic rats and across the monolayer of podocytes exposed to high glucose concentrations, with concomitant attenuation of HG-induced oxidative stress in podocytes. ( a ) ADAM17 inhibition in the presence of TAPI-0 improved glomerular albumin permeability ( n = 10). ( b ) ADAM17 inhibition ameliorated albumin permeability via the monolayer of podocytes ( n = 6) that were cultured in the presence of HG concentrations. ( c ) The elevation of ROS production in podocytes that were exposed to HG was reduced in the presence of TAPI-0 ( n = 8). ( d ) The induction of oxidative stress under HG conditions, manifested by an increase in NADPH oxidase activity, was attenuated by the ADAM17 inhibitor TAPI-0 ( n = 4). The results are expressed as the mean ± SD. **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05. Ctrl, control; STZ, streptozotocin; SG, standard glucose; HG, high glucose; TAPI-0, ADAM17 inhibitor.

Article Snippet: The ADAM17 inhibitor N -( R )-(2-[hydroxyaminocarbonyl]methyl)-4-methylpentanoyl-L-naphthylalanyl-L-alanine amide (TAPI-0) was purchased from Merck (catalog no. SML1292).

Techniques: Inhibition, Permeability, Cell Culture, Activity Assay, Control

Journal: International Journal of Molecular Sciences

Article Title: Involvement of ADAM17-Klotho Crosstalk in High Glucose-Induced Alterations of Podocyte Function

doi: 10.3390/ijms26020731

Figure Lengend Snippet: Silencing of ADAM17 gene expression attenuates the increase in its extracellular activity in podocytes exposed to HG medium. ( a ) ADAM17 mRNA levels decreased in shADAM17 cells ( n = 16). A representative agarose gel of PCR products is shown. ( b ) Analysis of immunofluorescence staining of ADAM17 in control podocytes (shCtrl) and cells with downregulated ADAM17 expression (shADAM17). The corrected total cell fluorescence was measured using ImageJ 4.1 software ( n = 7–11). Scale bar = 20 μm. ( c , d ) ADAM17 activity in ( c ) the lysate ( n = 8) and ( d ) extracellular medium ( n = 4) of shADAM17 cells compared with shCtrl cells that were cultured under SG or HG conditions. The results are expressed as the mean ± SD. **** p < 0.0001, ** p < 0.01. SG, standard glucose; HG, high glucose.

Article Snippet: The ADAM17 inhibitor N -( R )-(2-[hydroxyaminocarbonyl]methyl)-4-methylpentanoyl-L-naphthylalanyl-L-alanine amide (TAPI-0) was purchased from Merck (catalog no. SML1292).

Techniques: Expressing, Activity Assay, Agarose Gel Electrophoresis, Immunofluorescence, Staining, Control, Fluorescence, Software, Cell Culture

Journal: International Journal of Molecular Sciences

Article Title: Involvement of ADAM17-Klotho Crosstalk in High Glucose-Induced Alterations of Podocyte Function

doi: 10.3390/ijms26020731

Figure Lengend Snippet: ADAM17 downregulation remodels the actin cytoskeleton in podocytes and improves albumin permeability through the monolayer of podocytes cultured under high glucose conditions. The F-actin network was labeled with isothiocyanate phalloidin and visualized by fluorescence microscopy. Control cells (shCtrl) and cells with the silencing of ADAM17 gene expression (shADAM17) were grown on coverslips and incubated in ( a ) standard glucose (SG; 11 mM) or ( b ) high glucose (HG; 30 mM) medium. Scale bar = 25 μm. Digitized fluorescence images of the F-actin network were used to generate fluorescence intensity profiles (from the basal membrane to the nucleus) using NIS-Elements 5.11.02 imaging software ( n = 20). ( c ) Effects of ADAM17 downregulation on albumin permeability through the monolayer that was formed by shCtrl or shADAM17 podocytes that were cultured in an SG or HG environment ( n = 8). ( d ) The high glucose-induced activation of NADPH oxidase was attenuated in shADAM17 podocytes ( n = 6). The results are expressed as the mean ± SD. **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05. SG, standard glucose; HG, high glucose.

Article Snippet: The ADAM17 inhibitor N -( R )-(2-[hydroxyaminocarbonyl]methyl)-4-methylpentanoyl-L-naphthylalanyl-L-alanine amide (TAPI-0) was purchased from Merck (catalog no. SML1292).

Techniques: Permeability, Cell Culture, Labeling, Fluorescence, Microscopy, Control, Expressing, Incubation, Membrane, Imaging, Software, Activation Assay

Journal: International Journal of Molecular Sciences

Article Title: Involvement of ADAM17-Klotho Crosstalk in High Glucose-Induced Alterations of Podocyte Function

doi: 10.3390/ijms26020731

Figure Lengend Snippet: The suppression of Klotho gene expression promotes the shedding of ADAM17 in podocytes. ( a ) mRNA ( n = 6) and ( b ) protein ( n = 6) levels of Klotho in shKlotho podocytes. ( c ) ADAM17 protein levels in shKlotho podocytes ( n = 11). ( d , e ) Activity of ADAM17 in ( d ) shKlotho podocyte lysates ( n = 12) and ( e ) extracellular medium ( n = 5–6). The results are expressed as the mean ± SD. **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05.

Article Snippet: The ADAM17 inhibitor N -( R )-(2-[hydroxyaminocarbonyl]methyl)-4-methylpentanoyl-L-naphthylalanyl-L-alanine amide (TAPI-0) was purchased from Merck (catalog no. SML1292).

Techniques: Expressing, Activity Assay

Journal: International Journal of Molecular Sciences

Article Title: Involvement of ADAM17-Klotho Crosstalk in High Glucose-Induced Alterations of Podocyte Function

doi: 10.3390/ijms26020731

Figure Lengend Snippet: Klotho reduces the HG-induced elevation of ADAM17 extracellular activity in podocytes. ( a ) ADAM17 protein levels ( n = 5–6) and ( b ) intracellular distribution in podocytes that were cultured under SG or HG conditions in the presence of Klotho. Scale bar = 25 μm. ( c , d ) Activity of ADAM17 in ( c ) podocyte lysates ( n = 6) and ( d ) the extracellular medium ( n = 5) in the presence of Klotho during exposure to an HG environment. The results are expressed as the mean ± SD. *** p < 0.001, ** p < 0.01, * p < 0.05. SG, standard glucose; HG, high glucose.

Article Snippet: The ADAM17 inhibitor N -( R )-(2-[hydroxyaminocarbonyl]methyl)-4-methylpentanoyl-L-naphthylalanyl-L-alanine amide (TAPI-0) was purchased from Merck (catalog no. SML1292).

Techniques: Activity Assay, Cell Culture

Journal: International Journal of Molecular Sciences

Article Title: Involvement of ADAM17-Klotho Crosstalk in High Glucose-Induced Alterations of Podocyte Function

doi: 10.3390/ijms26020731

Figure Lengend Snippet: Proposed mechanism of the ADAM17-dependent detrimental effect on podocyte function induced by high glucose concentrations.

Article Snippet: The ADAM17 inhibitor N -( R )-(2-[hydroxyaminocarbonyl]methyl)-4-methylpentanoyl-L-naphthylalanyl-L-alanine amide (TAPI-0) was purchased from Merck (catalog no. SML1292).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Involvement of ADAM17-Klotho Crosstalk in High Glucose-Induced Alterations of Podocyte Function

doi: 10.3390/ijms26020731

Figure Lengend Snippet: Primers and probes used for real-time PCR.

Article Snippet: The ADAM17 inhibitor N -( R )-(2-[hydroxyaminocarbonyl]methyl)-4-methylpentanoyl-L-naphthylalanyl-L-alanine amide (TAPI-0) was purchased from Merck (catalog no. SML1292).

Techniques:

Journal: iScience

Article Title: Heightened innate immune state induced by viral vector leads to enhanced response to challenge and prolongs malaria vaccine protection

doi: 10.1016/j.isci.2024.111468

Figure Lengend Snippet:

Article Snippet: TAPI2 , Biosynth , Cat #INH-3852-PI.

Techniques: Control, Virus, Recombinant, Giemsa Stain, Cell Stimulation, Transfection, Software